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. 2015 Aug 14;43(19):9291–9305. doi: 10.1093/nar/gkv818

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© The Author(s) 2015. Published by Oxford University Press on behalf of Nucleic Acids Research.

This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by-nc/4.0/), which permits non-commercial re-use, distribution, and reproduction in any medium, provided the original work is properly cited. For commercial re-use, please contact journals.permissions@oup.com

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Figure 5. — Fluorescence intensity changes at 370 nm observed for the gp32 titration of 56-mer-ssDNA constructs with 2-AP monomer probes at various positions. (A) Fluorescence intensity changes of the DNA constructs (1 μM) upon the addition of various concentrations of gp32 per 56-mer ssDNA lattice. ₅₆F₄ construct (black curve, closed circles); ₅₆F₂₁ construct (green curve, open squares); and ₅₆F₅₃ construct (red curve, open circles). (B) Normalized fluorescence intensity changes of these DNA constructs (1 μM) upon addition of increasing concentrations of gp32 per ssDNA lattice. Same constructs and color-coding as in (A). The dashed vertical lines represent the expected saturation point at 8 gp32 monomers added per 56-mer ssDNA lattice.