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. 2015 Aug 14;43(19):9291–9305. doi: 10.1093/nar/gkv818

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© The Author(s) 2015. Published by Oxford University Press on behalf of Nucleic Acids Research.

This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by-nc/4.0/), which permits non-commercial re-use, distribution, and reproduction in any medium, provided the original work is properly cited. For commercial re-use, please contact journals.permissions@oup.com

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Figure 6. — Circular dichroism and fluorescence intensity changes observed for dsDNA constructs with 2-AP dimer or monomer probes site-specifically positioned within duplex DNA constructs. (A) CD spectra for a ₂₅B₁₀,₁₁–₂₅C dsDNA construct consisting of a ₂₅B_10,11 ssDNA lattice complexed to a fully complementary ₂₅C ssDNA lattice in the presence of increasing concentrations of gp32 monomers. blue: free DNA construct; green: 3; brown: 6; and olive green: 12 gp32 monomers present per dsDNA lattice. (B) Normalized (to unity) fluorescence intensity changes at 370 nm for 1 μM ₂₅B_10,11–25C and ₂₅B_11–25C dsDNA constructs plotted as a function of gp32 monomers per dsDNA construct. Construct ₂₅B_11–25C (open circles) and ₂₅B_10,11–25C (closed circles). The dashed vertical line marks the point at which one gp32 monomer has been added per dsDNA construct.