Figure - PMC

Skip to main content

An official website of the United States government

Here's how you know

Here's how you know

Official websites use .gov
A .gov website belongs to an official government organization in the United States.

Secure .gov websites use HTTPS
A lock ( ) or https:// means you've safely connected to the .gov website. Share sensitive information only on official, secure websites.

View full-text article in PMC

. 2015 Sep 10;43(19):9541–9552. doi: 10.1093/nar/gkv890

Search in PMC
Search in PubMed
View in NLM Catalog
Add to search

© The Author(s) 2015. Published by Oxford University Press on behalf of Nucleic Acids Research.

This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted reuse, distribution, and reproduction in any medium, provided the original work is properly cited.

PMC Copyright notice

Figure 6. — Anomeric structure of the abasic sugar observed in TDG enzyme-product complexes. (A) Abasic sites exist in several potential forms for enzyme-free DNA in solution. The α and β anomers of the cyclic hemiacetal predominate (together comprising 99%); minor forms are the ring-opened aldehyde and a hydrated aldehyde (not shown). For the TDG reaction, the initial enzyme-bound product resulting from C-N bond cleavage is expected to be the α anomer. (B) The α anomer is the predominant form of the abasic sugar observed for all TDG^cat product complexes that include an acetate molecule in the active site (crystallized in the presence of 0.3 M acetate). (C) A roughly equal mix of α and β anomers is observed in a TDG^cat product complex that lacks acetate in the active site. The β anomer appears to be stabilized by hydrogen bonds from C1’-OH to water molecules in the active site pocket.