Table 1. TP53 DNA sequences used for molecular dynamics simulations (adducted guanine underlined). Sequences were determined to be hotspots in cancers based on their prevalence in the IARC TP53 database, or the UMD TP53 database (5,33).

CODON	SEQUENCE	HOTSPOT STATUS (across cancers)	No. observed G:C>T:A in lung cancer *	P-value **
157	CCCGCGTCCGC	Lung	27	1 x 10−27
158	CGTCCGCGCCA	Lung	35	4 x 10−39
245	TGGGCGGCATG	Many	9	4 x 10−6
248	GAACCGGAGGC	Many	24	2 x 10−23
273	GGTGCGTGTTT	Many	36	1 x 10−40
282	AGACCGGCGCA	Many	1 (NS)	0.362
170	ATGACGGAGGT	None	0	0.303
186	ATAGCGATTGT	None	0	0.303
213	TTTTCGACATA	Breast	2 (1 NS)	0.216
267	GGGACGGAACA	None	0	0.303
290	TCTCCGCAAGA	None	2 (1 NS)	0.216

*Total number of observed G:C>T:A substitutions observed in the IARC TP53 database for codons 5–8 where smoking status was known.

**P-value denotes for each codon whether the number of G:C>T:A substitutions at the mutable guanine formed a significant mutation hotspot in lung cancer.

NS = Non-smoker.