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. 2015 Sep 22;43(19):9564–9572. doi: 10.1093/nar/gkv944

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© The Author(s) 2015. Published by Oxford University Press on behalf of Nucleic Acids Research.

This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted reuse, distribution, and reproduction in any medium, provided the original work is properly cited.

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Figure 2. — Single-point mutation (SPM) landscapes. (A) The normalized fluorescent signal of the Spinach mutant (S_m) to the Spinach wt (S_wt), (B) free energy (ΔΔG), (C) enthalpy (ΔΔH), and (D) Δ . of SPMs, where all values are relative to the WT Spinach. For each sequence positions of Spinach the two transversion and one transition mutation were included.

Inline graphic — Single-point mutation (SPM) landscapes. (A) The normalized fluorescent signal of the Spinach mutant (S_m) to the Spinach wt (S_wt), (B) free energy (ΔΔG), (C) enthalpy (ΔΔH), and (D) Δ . of SPMs, where all values are relative to the WT Spinach. For each sequence positions of Spinach the two transversion and one transition mutation were included.