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. 2015 Oct 1;43(19):9446–9456. doi: 10.1093/nar/gkv989

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© The Author(s) 2015. Published by Oxford University Press on behalf of Nucleic Acids Research.

This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted reuse, distribution, and reproduction in any medium, provided the original work is properly cited.

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Figure 2. — MALDI-MS analysis of digested tRNAs extracted from complemented Δdus2 yeast. MALDI-MS spectrum of RNAs fragments resulting from RNAse A digestion of bulk tRNAs originated from wild-type BY4741 (MATa; his3Δ 1; leu2Δ 0; met15Δ 0; ura3Δ 0) strain transformed by empty pCM190 vector (A), from Δdus2 BY4741 dus2::kanMX4 strain transformed by empty pCM190 (B) HsDus2-pCM190 (C) HsDus2^dusD-pCM190 (D) or by HsDus2^dsRBD-pCM190 vector (E). Peaks are identified by there m/z value and the corresponding trinucleotide obtained after RNAse A digest of bulk tRNA. T is ribothymidine, ′ is 1-methyladenosine, L is 2-methylguanosine, K is 1-methylguanosine and # is 2′-O-methylguanosine.