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Implication of HsDus2 domains in the binding of tRNA. The ability of HsDus2 (A), Saccharomyces cerevisiae Dus2p (B), HsDus2dsRBD (C), and HsDus2dusD (D) to form stable complexes with bulk tRNA was tested by gel shift assays. The first line corresponds to the tRNA alone while the following lines correspond to the tRNA (10 μM) mixed with increasing amounts of protein (1.06, 2.12, 4.25, 8.5, 17, 34, 68 and 136 μM).
