Figure 3. Stimulation of the self-renewing and senescent hADSCs with IL-2 upregulates mRNA of a mediator of IL-2 signaling STAT5 gene.

STAT5A and STAT5B mRNA expression was assessed by quantitative RT-PCR in un-stimulated (IL-2-) senescent (red) and self-renewing (blue) hADSCs and upon stimulation with 20ug/ml of recombinant IL-2 (IL-2+). Data shown as fold change ΔΔCT Mean ± SD from three independent experiments is shown. Position of the qPCR primers are depicted graphically. Statistical significance was estimated by t - test, where ***p < 0.001, **p < 0.01.