Figure 1. Mutp53 accumulation in morphologically normal multiple tissues in p53 R172H mice.

Adult p53 R172H mice were sacrificed in 2 mo and multiple tissues were harvested and embedded in paraffin block for immunostaining with p53 antibody. Tissues are divided into four groups according to p53 expression levels and positive cell fraction: mutp53 4+: small intestine, colon, rectum and thymus; mutp53 3+: bone marrow of vertebrae and femur, spleen, growing skin and hair follicle; mutp53 2+: kidney, nonglandular & glandular stomach and ependyma of brain; mutp53 1+: testis, pancreas & islet of Langerhan, lung, and cornea of eye. Mutp53 Ne (Negative): liver, brain (except ependyma) and skeletal muscle. Scale bar: 50 μm.