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. 2015 May 18;6(20):18012–18026. doi: 10.18632/oncotarget.4000

Figure 3. miR-23b inhibits growth and progression of gastric cancer cells.

Figure 3

A-C. NUGC-3 and AZ521 cells were infected with adenoviruses expressing miR-23b (Ad-miR-23b) or GFP (Ad-GFP) and SC-M1 cells were transfected with 100 nM antagomir-23b (anti-miR-23b) or scrambled control (−). The infected NUGC-3 (upper) as well as AZ521 cells (middle) and transfected SC-M1 cells (lower) were seeded and then counted by trypan blue exclusion method A or incubated to analyze cell viability by MTT assay B at the time indicated. The infected NUGC-3 as well as AZ521 cells (left) and transfected SC-M1 cells (right) were seeded for colony formation (upper), migration (middle), and invasion (lower) assays C Means of at least three independent experiments performed in triplicate are shown. *P < 0.05; **P < 0.01; ***P < 0.001. Data are shown as mean ± standard deviation.