Skip to main content
. 2015 May 18;6(20):18012–18026. doi: 10.18632/oncotarget.4000

Figure 7. miR-23b diminishes tumor growth and lung metastasis of SC-M1 gastric cancer cells via Notch2 pathway.

Figure 7

A-B. After infection with adenoviruses expressing miR-23b (Ad-miR-23b) or GFP (Ad-GFP) into SC-M1 cells A and SC-M1/myc-N2IC-His cells as well as SC-M1/pcDNA3 control cells B for 48 hours, the viable infected cells were subcutaneously injected into nude mice (n = 5 per group) for the measurement of tumor sizes at the time indicated (left). On day 27, the mice were sacrificed and subcutaneous tumors were excised for the detection of miR-23b expression using miRNA quantitative real-time PCR (right). Data are representative of 3 experiments. Bar, 1.0 cm. *P < 0.05; ***P < 0.001. C. For measurement of metastatic nodules in lungs, NOD-SCID mice (n = 6 per group) were injected with the viable SC-M1/myc-N2IC-His cells and SC-M1/pcDNA3 control cells by tail vein injection after infection with adenoviruses expressing miR-23b or GFP. After 15 weeks, the mice were sacrificed and the metastatic nodules in the lungs were counted by gross and microscopic examination. Data are from a representative experiment that was performed three times with identical results. **P < 0.01; #P < 0.05. Data are shown as mean ± standard deviation. D. A model depicting reciprocal regulation between Notch2 receptor and miR-23b in gastric carcinogenesis.