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. 2015 May 29;6(20):18116–18133. doi: 10.18632/oncotarget.4016

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Copyright: © 2015 Pesapane et al.

This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.

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A. Highly invasive HT1080 fibrosarcoma cells (upper panel) and MDAMB231 human breast cancer cells (lower panel) were treated with DMSO, with 10 μM (first line) and 5 μM (second line) of compounds NSC47923, NSC48478, NSC48861, NSC48869 and NSC210423, as a negative control, and allowed to invade Matrigel, using as chemoattractant cell culture medium supplemented with 10% FBS. Invading cells on the lower side of the membranes were fixed, stained and photographed: a representative image is shown. B. Highly invasive HT1080 fibrosarcoma cells (upper panel) and MDAMB231 human breast cancer cells (lower panel) were treated with DMSO (■), with 10 μM and 5 μM of compounds NSC47923 (□), NSC48478 (□), NSC48861 , NSC48869 , and NSC210423, as a negative control (□), and allowed to invade Matrigel, using as chemoattractant cell culture medium supplemented with 10% FBS. Invading cells on the lower side of the membrane were fixed, stained and counted and the results were expressed as a percentage of invasion in the absence of chemoattractant. The values are the mean ± SD of three experiments carried out in triplicate; (*, P < 0.05; **P < 0.01), as determined by the Student's t test. FBS-induced cancer cell invasiveness was significantly reduced by compounds NSC47923, NSC48478, NSC48861, NSC48869, at both concentrations tested.

Inline graphic — A. Highly invasive HT1080 fibrosarcoma cells (upper panel) and MDAMB231 human breast cancer cells (lower panel) were treated with DMSO, with 10 μM (first line) and 5 μM (second line) of compounds NSC47923, NSC48478, NSC48861, NSC48869 and NSC210423, as a negative control, and allowed to invade Matrigel, using as chemoattractant cell culture medium supplemented with 10% FBS. Invading cells on the lower side of the membranes were fixed, stained and photographed: a representative image is shown. B. Highly invasive HT1080 fibrosarcoma cells (upper panel) and MDAMB231 human breast cancer cells (lower panel) were treated with DMSO (■), with 10 μM and 5 μM of compounds NSC47923 (□), NSC48478 (□), NSC48861 , NSC48869 , and NSC210423, as a negative control (□), and allowed to invade Matrigel, using as chemoattractant cell culture medium supplemented with 10% FBS. Invading cells on the lower side of the membrane were fixed, stained and counted and the results were expressed as a percentage of invasion in the absence of chemoattractant. The values are the mean ± SD of three experiments carried out in triplicate; (*, P < 0.05; **P < 0.01), as determined by the Student's t test. FBS-induced cancer cell invasiveness was significantly reduced by compounds NSC47923, NSC48478, NSC48861, NSC48869, at both concentrations tested.