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. 2015 Jul 15;6(19):16981–16997. doi: 10.18632/oncotarget.4858

Figure 4. PAK1 regulates mTORC1 signaling independent of mTORC2-SGK1.

Figure 4

A. Immunoblotting of PAK1 shRNA Ben-Men-1 cells with indicated antibodies reveals that PAK1-suppression attenuates pS6 with no change in pNDRG1 levels compared to control shRNA. B. Immunoblot analysis of Ben-Men-1 cells treated with the group I PAK inhibitor FRAX597 shows a dose-dependent decrease in constitutive pS6 compared to DMSO (0). C. Immunoblot analysis with indicated antibodies Ben-Men-1 cells treated with FRAX597 (2 μM, 2h), or RAC1 shRNA reveals attenuation of both PAK1 (S144) phosphorylation (pPAK1, black arrowhead) and pS6, but no inhibition of pNDRG1 compared to control shRNA. Antibody for pPAK1 (S144) also detects pPAK2 (S141, gray arrowhead). NF2-expressing ACs serve as a control B. and C.. All experiments were carried out under serum-deprived conditions unless otherwise noted.