Table 2. Isogenic, clonally-derived lines generated from an immortalized arachnoid cell line using CRISPR-Cas9 genome editing.
| clone ID1 | NF2 expression | NF2 (ex8) mutation2 |
|---|---|---|
| A2 | + | wildtype |
| A3 | + | wildtype |
| A4 | − |
compound heterozygous alleles 1) 787del23bp, 263fs > 274X 2) 804insC, 269fs > 275X |
| A17 | − |
compound heterozygous alleles 1) 795del8bp, 265fs > 274X 2) 802insT, 268fs > 274X |
| A19 | − |
homozygous 787del35bp, E8/I8 |
1
ID numbers for NF2-expressing (+) and NF2-null (−) clones.
2
genotype for individual clones.