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. 2015 Apr 20;6(19):17039–17053. doi: 10.18632/oncotarget.3636

Figure 1. Stable overexpression of G3BP1 induces the EMT and enhances the migration and invasion of MDCK cells.

Figure 1

A. Morphological changes in MDCK cell lines stably expressing G3BP1 (G3BP1(+)-1 or G3BP1(+)-2) or an empty vector (Mock) (magnification, × 100). B. Western blot of whole-cell extracts from MDCK cells (Mock, G3BP1(+)-1 or G3BP1(+)-2) for G3BP1, E-cadherin, Keratin, Fibronectin, Vimentin, Snail, Slug and Zeb1. β-actin was used as a loading control. C. Transwell migration assay and Matrigel invasion assay using MDCK cells (Mock, G3BP1(+)-1 or G3BP1(+)-2) (magnification, × 100). *P < 0.05 versus Mock. The data represent the means±s.d. of three independent experiments. D. The expression of G3BP1, E-cadherin, Keratin19, Fibronectin, Vimentin and F-actin was detected via immunofluorescence staining (magnification, × 100).