Table 1. Computing simulation for obtaining the half maximal inhibitory concentration and the combination index in P19 cells treated with dichloroacetate (DCA) and melatonin (MEL).
| IC50(mM) | CI | |||
|---|---|---|---|---|
| MEL | DCA | 10 mM DCA + 0.1 mM MEL | 10 mM DCA + 1 mM MEL | |
| Glu-CSCs | n/a | 52.915 | n/a | n/a |
| Glu-dCCs | n/a | 9.955 | n/a | n/a |
| Gal-CSCs | 1.930 | 19.988 | 0.954 | 0.273 |
| Gal-dCCa | 0.024 | 14.360 | 2.052 | 0.496 |
Dose-response values IC50 (dose required for median effect) and the combination index (CI) were obtained with CompuSyn software for drug combinations and for general dose effect analysis, using data from cell mass studies in P19 embryonal carcinoma stem cells (CSCs) and differentiated cancer cells (dCCs) grown in glucose media (Glu) and in glucose-free galactose-containing media (Gal). The CI value reflects the extent of synergy or antagonism: CI < 0.9, synergy; 0.9 < CI < 1.1, additive effect; CI > 1.1, antagonism. n/a: not applicable.