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. 2015 May 8;6(19):17206–17220. doi: 10.18632/oncotarget.4043

Figure 3. YAP promotes CCA metastasis both in vitro and in vivo.

Figure 3

A. Wound-healing assay showed that overexpressing YAP promoted CCA cells migration, whereas silencing YAP inhibited migration. Representative images were taken at 0 and 48 hours after scratching. B. Representative images of invasion assays for the CCA cell lines. C. The number of invaded cells was counted in different cell lines. D. The multiple tumor masses (black arrows) formed by the HCCC9810-NC cells were much more than that by HCCC9810-LV-1 cells (top and middle panel). The median survival time of the nude mice zenografted with HCCC9810-NC or HCCC9810-LV-1 cells were 77 and 113 days, respectively (bottom panel). E. The multiple tumor masses (black arrows) formed by the QBC939-vector cells were much less than that by QBC939-YAP cells (top and middle panel). The median survival time of the nude mice zenografted with QBC939-vector cells or QBC939-YAP cells were 94 and 62 days, respectively (bottom panel). Experiments were done three times and data are presented as mean±SD. **P < 0.01; ***P < 0.001.