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. 2015 May 8;6(19):17206–17220. doi: 10.18632/oncotarget.4043

Figure 6. Gankyin upregulates YAP at transcriptional level and is responsible for YAP-induced oncogenic activity.

Figure 6

A. Relative expressions of gankyrin, LATS1, LATS2, YAP and p-YAP were detected by western blot in gankyrin knockdown and control cells. B. Relative expressions of gankyrin, LATS1, LATS2, YAP and p-YAP were detected by western blot analysis in Gankyrin overexpressed and control cells. C.-D. The mRNA level of YAP in gankyrin knockdown or overexpression cells compared to the control cells. E. RBE-YAP and QBC939-YAP cells were transfected with gankyrin siRNA, relative expressions of gankyrin, E-cadherin, N-cadherin were detected by Western blotting. F.-G. Colony formation and invasion assays were done for the QBC939-YAP cells following transfection with gankyrin siRNA. H. Schematic presentation of the mechanism underlying YAP-facilitated cholangiocarcinogenesis and metastasis. The results are presented as mean±SD from three independent experiments. ***P < 0.001.