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. 2015 Apr 29;6(19):17221–17236. doi: 10.18632/oncotarget.3948

Figure 3. TRPM8 expression suppresses cell viability through the regulation of AR in PC cells.

Figure 3

A.-B. FACS analysis of cell cycle progression in DHT and testosterone-induced LNCaP cells both in the A. absence and B. presence of hydroxyflutamide (HF). The cells were then treated with menthol for 30 min and re-analyzed by FACS. C. The cell cycle progression in control and TRPM8OE PC3 cells. The cells were then treated with menthol/testosterone for 30 min and re-analyzed by FACS. The percentages of cells in sub-G1 (M1), G0/G1 (M2), S (M3) and G2/M (M4) phases of cell cycle were calculated using CellQuest Pro software and graphically represented. D. The PC3 cells transiently transfected with GFP-tagged TRPM8 E.-F. Clonogenic assay for E. LNCaP and F. PC3 cells. The number of colonies was quantified as the measure of clonogenicity. G. Transmission electron micrographs of nucleus in control and TRPM8 overexpressing PC3 cells. Images are representatives of three experiments (n = 3). TRPM8 overexpressing PC3 cell show blebbing of nuclear membrane (depicted by arrows).