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. 2013 Aug 12;6(19):17314–17327. doi: 10.18632/oncotarget.1168

Figure 2. CHR-3996 induces cell cycle arrest and apoptosis via caspase-dependent and independent pathways.

Figure 2

A. H929 or RPMI-8226 cells were treated with CHR-3996 at 250 or 100 nM respectively for 48 hours, following which the cells were fixed in alcohol, treated with RNase, then stained with Propidium Iodide (PI) and analysed by flow cytometry. B. H929 (i) and RPMI-8226 (ii) cells were treated with CHR-3996 at 250 or 100 nM respectively and cells lysed for protein at 8, 24, and 48 hours and used for immunoblotting. C. H929 cells were pre-treated with Z-VAD-FMK (50 μM) for 1.5 hours prior to addition of varying concentrations of CHR-3996 (250 nM). The cells were harvested and stained with annexinV and PI and analysed by flow cytometry. Cells staining positive for annexinV alone or annexinV and PI were defined as apoptotic. A representative of three experiments is shown.