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. 2015 May 11;6(19):17532–17542. doi: 10.18632/oncotarget.3947

Figure 2. Caspase inhibition (A) caspase-8 knockdown (B) or FADD deficiency (C and D) protects cancer cells from CFZ-induced apoptosis.

Figure 2

A, The indicated cell lines were treated with 100 nM CFZ in the absence or presence of 20 μM Z-VAD-FMK for 24 h and then harvested for preparation of whole-cell protein lysates and subsequent Western blot analysis. B, HCT116 cells were transfected with the indicated siRNAs and then after 36 h, were exposed to 100 nM CFZ for an additional 24 h. The cells were harvested for preparation of whole-cell protein lysates and subsequent Western blot analysis. C, The indicated WT and FADD-KO cell lines were treated with 100 nM CFZ for 8 h and then harvested for preparation of whole-cell protein lysates and subsequent Western blot analysis to confirm FADD expression. The indicated cell lines were also treated with different concentrations of CFZ for 48 h and then subjected to estimation of cell number with the SRB assay. The data are means ± SDs of four replicate determinations. D, The given cell lines were treated with the indicated concentrations of CFZ for 17 h and then subjected to preparation of whole-cell protein lysates and subsequent Western blot analysis. CF, cleaved fragment.