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. 2015 Apr 22;6(19):17685–17697. doi: 10.18632/oncotarget.3779

Figure 2. TEAD4 overexpression promotes TNBC cell proliferation and tumor growth.

Figure 2

A. Stable overexpression of TEAD4 decreased the p27 protein expression level in HCC1937. B. TEAD4 overexpression significantly promoted HCC1937 cell growth, as determined by the SRB assay. **p < 0.01, t-test. C. TEAD4 overexpression significantly (p < 0.01) increased DNA synthesis in HCC1937 cells, as measured with a Click-iT EdU Alexa Fluor Imaging Kit. D. TEAD4 overexpression significantly increased G1/S cell cycle transition in HCC1937 cells, as measured by PI staining and flow cytometry. a&c, the cells were arrested at the G1 phase by serum starvation. b&d, the arrested cells were stimulated with 10% serum for 4 hours. E. TEAD4 promotes cell proliferation in part through KLF5 in HCC1937 cells. KLF5 was transiently knocked down in HCC1937-pBabe and HCC1937–pBabe-TEAD4 cells. Western blot results are shown on the left side and cell viability data are shown on the right side. **, p < 0.01, n.s., not significant, t-test. F. Tumor masses harvested from HCC1937-pBabe and –pBabe-TEAD4 after tumors had grown for 6 weeks. TEAD4 overexpression significantly increases xenograft weight (right panel). G. TEAD4 overexpression significantly promotes tumor growth in female NOD-SCID mice.