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. 2015 Apr 22;6(19):17685–17697. doi: 10.18632/oncotarget.3779

Figure 4. TEAD4 and KLF5 promote cell proliferation partially by suppressing the p27 gene transcription together in TNBC cell lines.

Figure 4

A. Knockdown of TEAD4 by two different siRNAs upregulated p27 mRNA levels in both HCC1937 and HCC1806, as measured by qRT-PCR. B. Knockdown of KLF5 by two different siRNAs upregulated p27 mRNA levels in both HCC1937 and HCC1806, as measured by qRT-PCR. C. Knockdown of TEAD4 and KLF5 activated the p27 gene promoter in HCC1806 cells. TEAD4 and KLF5 knockdown were evaluated by WB. Luciferase activities were measured by the dual-luciferase assay kit. D. Both TEAD4 and KLF5 bind to the p27 gene promoter as determined by ChIP assays in HCC1806. E. Knockdown of p27 rescued the TEAD4 and KLF5 knockdown-induced growth arrest in HCC1806, as determined by the SRB assay. **p < 0.01, t-test. F. Knockdown of KLF5 and TEAD4 together additively increased the p27 protein levels in HCC1806.