Figure 3. c-MYC, BRD2/3/4 and HEXIM1 expression in AML and ALL cell lines after OTX015 treatment.

A. c-MYC basal gene expression in AML and ALL cell lines determined by RT-qPCR, relative to ABL 10². Results are shown as mean +/− SEM from duplicates of three independent experiments. B. Western blot showing c-MYC, BRD2/3/4, and HEXIM1 protein changes in OCI-AML3 and JURKAT cells treated with 500nM OTX015 for 24, 48 or 72h or 0.1% DMSO. GAPDH was used as a loading control. One representative experiment out of three is shown. C. RT-qPCR showing c-MYC decrease in AML and ALL cell lines after 4 and 24h exposure with 500nM OTX015, relative to GAPDH and normalized to 0.1% DMSO. Results are shown as mean +/− SEM from duplicates of three independent experiments. D. RT-qPCR showing BRD4, BRD2, and BRD3 basal gene expression in leukemia cell lines, relative to ABL 10². Results are shown as mean +/− SEM from duplicates of three independent experiments. E. RT-qPCR showing BRD4, BRD2, and BRD3 mRNA expression levels after 48h exposure to 500nM OTX015 in leukemia cell lines, relative to ABL and normalized to 0.1% DMSO. Results are shown as mean +/− SEM from duplicates of three independent experiments. F. RT-qPCR showing HEXIM1 mRNA increase in AML and ALL cell lines after 4h and 24h exposure with 500nM OTX015, relative to GAPDH and normalized to 0.1% DMSO. Results are shown as mean +/− SEM from duplicates of three independent experiments.