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. 2015 Apr 23;6(19):17738–17752. doi: 10.18632/oncotarget.3921

Figure 2. Effect of lapatinib on the expression of ABCB1.

Figure 2

The protein level of ABCB1 was measured by Western blot and mRNA level was measured by qRT-PCR. A. B. Lapatinib did not alter the protein levels or mRNA levels in HepG2/Adr cells. C. Grayscale ratios of ABCB1/GAPDH were analyzed with Image J. The grayscale ratios were proportional to the ABCB1 protein levels. D. qRT-PCR was further applied to confirm unchangeable mRNA levels in HepG2/Adr cells. All experiments were repeated at least three times, and a representative set of data is shown in each panel.