Skip to main content
. 2015 Jun 23;6(19):17805–17816. doi: 10.18632/oncotarget.4596

Figure 3. MiR-31 inhibits TRADD expression and NF-κB-induced gene expression.

Figure 3

A.-D. U87-MG cells were transfected with control (CT) miR or miR-31. A. TRADD mRNA and protein levels were evaluated by qRT-PCR and immunoblot analyses, respectively. (**, p < 0.005). B., C. The levels of IL-6 mRNA B. and protein C. in U87-MG cells as described in A., were evaluated by qRT-PCR and ELISA, respectively. (**, p < 0.005). D. U87-MG cells were transfected with CT or miR-31, and grown in the absence or presence of TNF-α (10 ng/ml). IL-6 protein levels were evaluated by ELISA. (*, p < 0.05; **, p < 0.005). E., F. U87-MG cells were transfected with CT or miR-31, and either a control plasmid (CT) or a plasmid expressing TRADD, but lacking the 3′ UTR. MiR-31 E. and IL-6 F. RNA levels were measured by qRT-PCR. (**, p < 0.005).