Table 5.
Effect of ionizing radiation on the phosphorylation of RNA polymerase II CTD peptides
| Peptide | Phosphorylation site | No IR | IR 2 h | No IR/2 h IR |
|---|---|---|---|---|
| YSPTSPTphosYSphosPTphosSPK | [1915, 1917, 1919] | 2.33E+06 | 4.43E+06 | 0.53 |
| YSPTSPTYphosSPTphosSPK | [1916, 1919] | 2.79E+06 | 1.71E+06 | 1.63 |
| YSPTphosSPTYSphosPTphosSPK | [1912, 1917, 1919] | 2.57E+06 | 3.48E+06 | 0.74 |
| YSPTSphosPTYSphosPTphosSPK | [1913, 1917, 1919] | 3.32E+06 | 6.08E+06 | 0.55 |
| YphosSPTSPTYphosSPTTPK | [1874, 1881] | 8.42E+05 | 9.24E+05 | 0.91 |
| YphosSPTSPTYSphosPTTPK | [1874, 1882] | 8.08E+05 | 5.88E+05 | 1.37 |
| YSPTSPTYSphosPTphosSPK | [1917, 1919] | 4.96E+06 | 3.16E+06 | 1.57 |
| YSPTSphosPTYSPTphosSPK | [1913, 1919] | 4.76E+06 | 7.32E+06 | 0.65 |
| YSPTSPTYSphosPTSphosPK | [1917, 1920] | 1.00E+07 | 6.01E+06 | 1.66 |
| YSPTSphosPTYSphosPTSphosPK | [1913, 1917, 1920] | 3.80E+06 | 7.93E+06 | 0.48 |
| YSPTSphosPTYSPTphosSphosPK | [1913, 1919, 1920] | 2.33E+06 | 4.43E+06 | 0.53 |
| YSPTSphosPTYSphosPTSPK | [1913, 1917] | 2.54E+06 | 7.72E+05 | 3.29 |
| YSPTSPTYphosSPTSphosPK | [1916, 1920] | 4.33E+06 | 2.87E+06 | 1.51 |
| YphosSPTSPTYSPTSphosPK | [1909, 1920] | 1.40E+06 | 1.00E+06 | 1.4 |
| YSPTSPTphosYphosSPTSPK | [1915, 1916] | 4.90E+06 | 2.85E+06 | 1.72 |
| YSPTphosSPTphosYSPTphosSPK | [1912, 1915, 1919] | 1.42E+06 | 1.97E+06 | 0.72 |
| YSPTSphosPTYSPTSphosPK | [1913, 1920] | 4.76E+06 | 7.32E+06 | 0.65 |
| YSPTphosSPTYSphosPTSphosPK | [1912, 1917, 1920] | 3.50E+06 | 4.90E+06 | 0.71 |
| YSPTSPTphosYSphosPTSphosPK | [1915, 1917, 1920] | 2.70E+06 | 5.23E+06 | 0.52 |
| YphosSPTSPTYSphosPTSphosPK | [1909, 1917, 1920] | 3.32E+06 | 6.08E+06 | 0.55 |
| Peptide | Phosphorylation site | 30 min IR | 2 h IR | 30 min IR/2 h IR |
|---|---|---|---|---|
| YphosSPTSPTYphosSPTTPK | [1874, 1881] | 5.91E+05 | 1.19E+06 | 0.5 |
| YSphosPTSPTYphosSPTTPK | [1875, 1881] | 9.93E+05 | 1.49E+06 | 0.67 |
| YSPTphosSPTYphosSphosPTSPK | [1912, 1916, 1917] | 2.22E+06 | 1.22E+06 | 1.82 |
| YSPTphosSphosPTYSPTphosSPK | [1912, 1913, 1919] | 2.23E+05 | 1.26E+05 | 1.77 |
| YphosSPTSPTYSphosPTSphosPK | [1909, 1917, 1920] | 2.06E+06 | 3.53E+06 | 0.58 |
| YphosSPTSPTphosYSPTSphosPK | [1909, 1915, 1920] | 2.60E+06 | 4.02E+06 | 0.65 |
| YSPTSPTYphosSPTphosSPK | [1916, 1919] | 5.65E+06 | 4.15E+06 | 1.36 |
| YSPTSPTYSPTphosSphosPK | [1919, 1920] | 5.45E+06 | 4.05E+06 | 1.35 |
| YSPTphosSPTYSphosPTphosSPK | [1912, 1917, 1919] | 2.60E+06 | 4.02E+06 | 0.65 |
| YSPTSphosPTYSphosPTphosSPK | [1913, 1917, 1919] | 2.60E+06 | 4.02E+06 | 0.65 |
| YSPTSPTYphosSphosPTSPK | [1916, 1917] | 5.08E+06 | 3.53E+06 | 1.44 |
| YSPTSPTYSphosPTphosSPK | [1917, 1919] | 5.65E+06 | 4.15E+06 | 1.36 |
| YSPTSphosPTYSPTphosSPK | [1913, 1919] | 3.14E+06 | 6.77E+06 | 0.46 |
| YSPTSPTYSphosPTSphosPK | [1917, 1920] | 4.44E+06 | 3.47E+06 | 1.28 |
| YSPTSphosPTYSphosPTSphosPK | [1913, 1917, 1920] | 9.64E+06 | 2.73E+07 | 0.35 |
| YSPTSphosPTYSPTphosSphosPK | [1913, 1919, 1920] | 9.64E+06 | 2.73E+07 | 0.35 |
| YSPTphosSPTYSPTphosSphosPK | [1912, 1919, 1920] | 1.39E+06 | 1.93E+06 | 0.72 |
| YphosSPTSphosPTYSPTTPK | [1874, 1878] | 7.10E+05 | 1.48E+06 | 0.48 |
| YSPTSPTphosYphosSPTSPK | [1915, 1916] | 5.08E+06 | 3.53E+06 | 1.44 |
| YSphosPTSphosPTYSPTTPK | [1875, 1878] | 6.09E+05 | 1.30E+06 | 0.47 |
| YSPTSphosPTYSPTSphosPK | [1913, 1920] | 4.70E+06 | 9.61E+06 | 0.49 |
| YSphosPTSPTYSphosPTSphosPK | [1910, 1917, 1920] | 4.08E+06 | 7.53E+06 | 0.54 |
| YphosSPTSPTphosYSphosPTSPK | [1909, 1915, 1917] | 2.24E+06 | 1.30E+06 | 1.72 |
| YSPTphosSPTYSphosPTSphosPK | [1912, 1917, 1920] | 3.71E+06 | 1.01E+07 | 0.37 |
| YSPTphosSPTYphosSphosPTTPK | [1877, 1881, 1882] | 1.69E+06 | 1.78E+06 | 0.95 |
| YSPTphosSphosPTYphosSPTSPK | [1912, 1913, 1916] | 2.52E+06 | 1.19E+06 | 2.12 |
| Peptide | Phosphorylation site | 1 h IR | 2 h IR | 1 h IR/2 h IR |
|---|---|---|---|---|
| YphosSPTSphosPTYSPTSphosPK | [1909, 1913, 1920] | 4.69E+06 | 1.98E+06 | 2.37 |
| YphosSPTSPTphosYSPTSphosPK | [1909, 1915, 1920] | 2.59E+06 | 3.55E+06 | 0.73 |
| YSPTSPTYphosSPTphosSPK | [1916, 1919] | 5.08E+06 | 3.58E+06 | 1.42 |
| YSPTSphosPTYSphosPTphosSPK | [1913, 1917, 1919] | 1.81E+06 | 2.72E+06 | 0.67 |
| YphosSPTSPTYphosSPTTPK | [1874, 1881] | 6.79E+05 | 9.01E+05 | 0.75 |
| YSphosPTSPTYphosSPTTPK | [1875, 1881] | 7.12E+05 | 1.10E+06 | 0.65 |
| YSPTSPTYSphosPTphosSPK | [1917, 1919] | 6.85E+06 | 4.61E+06 | 1.49 |
| YSPTSphosPTYSPTphosSPK | [1913, 1919] | 3.20E+06 | 4.32E+06 | 0.74 |
| YSPTSPTYSphosPTSphosPK | [1917, 1920] | 6.85E+06 | 4.61E+06 | 1.49 |
| YSPTSphosPTYSphosPTSphosPK | [1913, 1917, 1920] | 9.32E+06 | 2.01E+07 | 0.46 |
| YSPTSphosPTYSPTphosSphosPK | [1913, 1919, 1920] | 3.70E+06 | 7.89E+06 | 0.47 |
| YphosSPTSPTYSPTSphosPK | [1909, 1920] | 3.78E+06 | 5.11E+06 | 0.74 |
| YSPTSphosPTYphosSPTTPK | [1878, 1881] | 5.57E+05 | 2.99E+05 | 1.86 |
| YSPTphosSPTYphosSPTTPK | [1877, 1881] | 7.12E+05 | 1.10E+06 | 0.65 |
| YSPTSPTphosYphosSPTSPK | [1915, 1916] | 1.27E+06 | 7.11E+05 | 1.79 |
| YSPTSphosPTYSPTSphosPK | [1913, 1920] | 3.78E+06 | 5.11E+06 | 0.74 |
| YSphosPTSPTYSphosPTSphosPK | [1910, 1917, 1920] | 1.58E+06 | 3.75E+06 | 0.42 |
| YSPTphosSphosPTYphosSPTSPK | [1912, 1913, 1916] | 1.30E+06 | 7.23E+05 | 1.8 |
| YSPTphosSPTYSphosPTSphosPK | [1912, 1917, 1920] | 4.90E+06 | 1.06E+07 | 0.46 |
| YSPTphosSPTphosYSPTSphosPK | [1912, 1915, 1920] | 4.56E+06 | 6.41E+06 | 0.71 |
| YSPTSPTphosYSphosPTSphosPK | [1915, 1917, 1920] | 1.61E+06 | 2.42E+06 | 0.67 |
Lists CTD phospho-peptides identified in the SILAC MS analyses of partially purified RNA polymerase II from HeLa cells exposed to ionizing radiation (IR, 8 Gy) for the indicated time. The peptide column lists phosphopeptides identified. Amino acid to the left of “phos” is phosphorylated. The phosphorylation sites in each peptide are listed in the second column. The signals and the ratio from light and heavy peptides are listed in the remaining columns. A ratio >1 represents a reduction in that phospho-peptide at 2 h relative to 0 h, 30 min or 1 h after IR. A ratio <1 represents an increase in that phospho-peptide at 2 h relative to 0 h, 30 min or 1 h after IR