Fig. 2.
Exosome-mediated miR-122 communication between AMSCs and HepG2 cells. a Western blot for CD9, CD63, and CD81 expression in AMSC-derived exosomes. b–d Real-time PCR detection of miR-122 expression in AMSCs (b), AMSC-derived exosomes (c), and exosome-treated HepG2 cells (d). e, f Confocal images of AMSC-122 stained with DilC16(3), a phospholipid membrane dye. Transfer of fluorescent exosomes from AMSC-122 is apparent in HepG2 cell membranes and cytoplasm. Data are presented as means ± SE. (*p < 0.05, n = 3). AMSC-122 miR-122-transfected AMSC, AMSC-67 cel-miR-67-transfected AMSC, Media precipitates from AMSC-conditioned media, Exo naïve AMSC-derived exosomes, 122-Exo AMSC-122-derived exosomes, 67-Exo AMSC-67-derived exosomes. Scale bar = 20 μm