Figure 2.

Representative micrographs of parasympathetic fibers innervating the extraorbital lacrimal gland in naïve animals. (A) Beta-tubulin-immunoreactive (-ir) fibers (red) run throughout the extraorbital lacrimal gland. (B) Vesicular acetylcholine transporter-immunoreactive fibers (green) are also present throughout the extraorbital lacrimal gland. (C) The vast majority of beta-tubulin-ir fibers also contain VAChT immunoreactivity (arrowheads), although there were a few examples of beta-tubulin-ir fibers that did not contain VAChT (arrow). Images presented are different channels from one 9 μm confocal projection. Scale bar: 20 μm.