Figure 1. Loss of usp7 Represses Hh Signaling and Decreases Ci Protein Level.

All wing imaginal discs shown in this study were oriented with anterior on the left and ventral on the top.

(A-A’) UAS-GFP (green) marks the apG4-mediated gene expression pattern. apG4 drives UAS transgenes to be specifically expressed in the dorsal region of wing discs.

(B-B’) Knockdown of usp7 by apG4 attenuated the expression of dpp-lacZ (arrow).

(C-J’) Knockdown of usp7 with apG4 attenuated the expression of ptc-lacZ (compared D-D’ with C-C’), kn-lacZ (compared F-F’ with E-E’), En (compared H-H’ with G-G’) and Ci (compared J-J’ with I-I’). Arrows indicate the decrease of ptc-lacZ, kn-lacZ, En and Ci.

(K-L”) Low (K) and high (L-L”) magnifications of wing disc carrying usp7^KG06814 clones was immunostained to show the expression of GFP (green) and Ci (red). usp7^KG06814 clones are recognized by the lack of GFP.

(L-L”) are enlarged views of the region marked by dashed lines in (K).

(M-N”) Wing discs carrying usp7^KG06814 clones were immunostained to show the expression of GFP (green) and En (white). The clones in A compartment near A/P boundary showed decrease of En (M-M”, arrows), while the clones in P compartment did not show En decrease (N-N”).

(O-P”) usp7^KG06814 clones showed a decrease of ptc-lacZ expression (arrow). (P-P”) are enlarged views of the region marked by dashed lines in (O).

See also Figure S1.