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. 2012 Apr 24;53(4):2208–2217. doi: 10.1167/iovs.11-8447

Figure 6.

Figure 6

Enhanced suppression of TNF-α–induced IL-6 and Ccl2 expression by NA and β-HB in GPR109A-overexpressing ARPE-19 cells. GPR109A was overexpressed in ARPE-19 cells using a lentiviral vector expression system. (A) RT-PCR was used to evaluate GPR109A expression in GPR109A-overexpressing (GPR109A-LV) and control ARPE-19 cells. (B) cAMP assay of GPR109A-LV and control ARPE-19 cells treated with FSK (10 μM) and/or NA (1 mM). qPCR was used to evaluate (C) Ccl2 and (D) IL-6 mRNA expression in GPR109A-LV and control ARPE-19 cells following exposure to TNF-α in the presence or absence of NA (1 mM) or β-HB (5 mM). *P < 0.01 compared with control cells; **P < 0.01 compared with FSK- or TNF-α–treated cells; #P < 0.05 compared with FSK- or TNF-α–treated control ARPE-19 cells.