Figure 2.

Further optimization of electroporation of GM12878 cell transfection efficiency by use of GFP-expressing plasmids and immunoblotting. A) GM12878 cells (4 × 10⁶) were transfected with 0.8 μg GFP-expressing plasmid by use of Lonza Kit V and Kit R with the indicated channels. The cells were stained 24 h after electroporation with Hoechst nuclear stain (1 μg/ml) for 1 h. All cells were imaged with an inverted fluorescence microscope, and the percentage of GFP-expressing cells was quantified (lower). B) Transfection efficiency was measured by protein immunoblot analysis by use of anti-GFP antibodies. The GFP expression levels were normalized by comparison of β-actin protein levels as a loading control.