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. 2015 Sep 3;19(11):2549–2563. doi: 10.1111/jcmm.12667

Figure 6.

Figure 6

Compstatin inhibits sepsis-induced expression of TIMP1. (A) Log ratio qPCR data showing transcript regulation of TIMP1 by sepsis and complement inhibition. Expression values were normalized by housekeeping gene β-actin. Data are shown as mean ± S.E.M. of three replicates. One-way anova with Dunnett's multicomparison test; ***P < 0.001 as compared to Escherichia coli versus Control ratio. (B) TIMP1 levels in citrated plasma at 24 hrs after challenge with E. coli with/without compstatin treatment during the first (T0) and second (T+5) stages of experimental sepsis. Data are shown as mean ± S.E.M. of three replicates. One-way anova with Dunnett's multicomparison test; ***P < 0.001, *P < 0.05 as compared to Control or E. coli groups. (C) Immunofluorescent staining and semi-quantitative image analysis (D) confirm the induction of TIMP1 by sepsis and the inhibitory effect of compstatin on this protease inhibitor. Magnification: bar, 50 μm. Data are shown as means ± S.E.M.; one-way anova with Dunnett's multicomparison test. ***P < 0.001, **P < 0.01, as compared to the E. coli group.