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. 2015 Sep 25;29(11):1558–1570. doi: 10.1210/me.2015-1145

Figure 2.

Figure 2.

CAR reduces the recruitment of PGC1α to the gluconeogenic gene promoters and causes redistribution of PGC1α to PML-NBs. A and B, Mouse primary hepatocytes were infected with Ad-HA-PGC1α and/or Ad-CAR for 48 hours, in the absence or presence of TCPOBOP (TC) (500nM). PGC1α (A) and HNF4α (B) ChIPs were facilitated by using anti-HA and anti-HNF4α antibodies, respectively (**, P < .01; N.S., not significant). C and D, Mouse primary hepatocytes were infected with Ad-PGC1α and/or Ad-CAR for 48 hours, in the absence or presence of TC (500nM) before immunofluorescent detection of CAR and PGC1α (C) or PML and PGC1α (D). E, 293T cells were cotransfected with Myc-PML, Flag-PGC1α, and HA-CAR before subjecting to immunoprecipitation and immunoblotting as indicated.