Figure 3.
Persistent cAMP signaling by MTII is not antagonized by AgRP. A and B, N2AHA-MC4R-GFP cells were transfected with TEpacVV. Cells were treated with α-MSH and MTII followed by a wash step where the medium was replaced for a total of 6 times before the addition of AgRP. Changes in corrected FRET ratio were monitored as in Figure 2C, normalized to that observed in response to the initial exposure to the agonist (Materials and Methods), and shown in the graphs as averages and SDs (n cells per condition = 5). Experiments in A and B were run in parallel using the same population of transfected cells.