Table 2.
LMM metal complexes and P and S compounds in mitochondria.
| Peak Name | Center (mL) | Width (mL) | Molecular Mass (Da) | [yeast] (μM) | [mouse] (μM) | [Jurkat] (μM) | R-value (%) |
|---|---|---|---|---|---|---|---|
| P1000 | 33.4 ± 0.2 | 0.9 ± 0.1 | 1000 ± 200 | 400 ± 100 | 500 | 400 | 100 |
| P800 | 34.6 ± 0.1 | 1.3 ± 0.2 | 800 ± 200 | 300 ± 100 | 400 | 400 | 100 |
| P520 | 37.1 ± 0.2 | 0.7 ± 0.1 | 520 ± 100 | 20000 ± 4000 | 24500 | 24000 | 100 |
| P200 | 42.7 ± 0.2 | 1.4 ± 0.1 | 200 ± 50 | 230 ± 140 | 100 | 100 | 80 |
| S1100 | 32.8 ± 0.3 | 1.0 ± 0.1 | 1100 ± 150 | 4900 ± 39 | 6300 | 6300 | 100 |
| S780 | 34.8 ± 0.1 | 1.4 ± 0.5 | 780 ± 200 | 500 ± 400 | n.d. | n.d. | 60 |
| Mn2000 | 30.5 ± 0.2 | 1.2 ± 0.1 | 2000 ± 200 | n.d. | 2 | 3.5 | n.d. |
| Mn1100 | 32.6 ± 0.1 | 0.9 ± 0.1 | 1100 ± 160 | 1.9 ± 0.2 | 2 | 0.5 | 100 |
| Fe1500 | 30.8 ± 0.1 | 0.8 ± 0.2 | 1500 ± 300 | n.d. | 15 | n.d. | n.d. |
| Fe1100 | 32.7 ± 0.1 | 1.3 ± 0.2 | 1100 ± 250 | 6 ± 2 | 10 | n.d. | 80 |
| Fe870 | 34.1 ± 0.5 | 0.7 ± 0.1 | 870 ± 100 | 12 ± 9 | 2 | n.d. | 60 |
| Fe680 | 35.6 ± 0.4 | 1.1 ± 0.4 | 680 ± 130 | 20 ± 10 | 2 | n.d. | 50 |
| Fe580 | 36.6 ± 0.3 | 1.4 ± 0.1 | 575 ± 150 | 90 ± 20 | 26 | 110 | 100 |
| Fe200 | 43.3 ± 0.2 | 1.2 ± 0.8 | 200 ± 40 | 2 ± 1 | 1 | n.d. | 90 |
| Co1500 | 30.8 ± 0.3 | 0.8 ± 0.2 | 1500 ± 300 | n.d. | 0.01 | 0.02 | 90 |
| Co1200 | 32.3 ± 0.2 | 0.9 ± 0.1 | 1200 ± 180 | 0.03 ± 0.02 | 0.04 | 0.04 | 90 |
| Co840 | 34.3 ± 0.3 | 1.7 ± 0.6 | 840 ± 270 | 0.02 ± 0.01 | 0.01 | 0.02 | 90 |
| Co450 | 38.2 ± 0.5 | 2.4 ± 0.9 | 450 ± 200 | 0.013 ± 0.22 | n.d. | n.d. | 70 |
| Co340 | 39.8 ± 0.2 | 1.8 ± 0.4 | 340 ± 100 | 0.006 ± 0.004 | n.d. | n.d. | 90 |
| Co300 | 41.1 ± 0.4 | 2.0 ± 1.5 | 300 ± 10 | 0.002 ± 0.001 | n.d. | n.d. | 80 |
| Cu5000 | 23.1 ± 0.1 | 1.4 ± 0.2 | 5000 ± 1000 | 16 ± 7 | n.d. | n.d. | 90 |
| Zn1500 | 30.8 ± 0.2 | 0.7 ± 0.1 | 1500 ± 0.2 | n.d. | 8 | 6 | n.d. |
| Zn1200 | 32.2 ± 0.2 | 0.6 ± 0.5 | 1150 ±100 | 110 ± 20 | 100 | 104 | 100 |
| Zn900 | 34.1 ± 0.1 | 1.8 ± 1.3 | 900 ± 300 | 6 ± 1 | n.d. | n | 100 |
| Mo730 | 35.0 ± 0.1 | 0.7 ± 0.1 | 730± 100 | n.d. | 0.4 | 0.3 | 100 |
Values were obtained by fitting to Gaussian functions as described in the text. n.d. means not detected. R-value indicates confidence in yeast traces only.