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. Author manuscript; available in PMC: 2015 Oct 30.
Published in final edited form as: Neuron. 2009 Jan 15;61(1):113–125. doi: 10.1016/j.neuron.2008.10.049

Figure 5. MAP kinase is required for AD-ITF in SN-MN culture.

Figure 5

AD-ITF induced by either (A) SN activity (4 trains 10Hz, 2 sec) with 5 min 5HT (10 µM) or (B) 5 min 100mM KCl-ASW + 10µM 5HT was blocked by the MEK inhibitor U0126. (A) Sample traces of EPSPs before and 30 min after AD-ITF induction in the presence of U0124 (inactive analog) or U0126. Scale bar: 20mV, 25ms. Histogram of group data shows that U0126 significantly blocked AD-ITF (t-test, p<0.05: n=4 for each group). (B) U0126 specifically blocked KCl+5HT induced ITF. Sample traces of EPSPs before and after KCl+5HT stimulation with U0126 or U0124. Scale bar: 20mV, 25ms. Histogram of group data shows that U0126 significantly blocks AD-ITF induced by KCl+5HT (t-test, p<0.05; n=20 for U0124 and 12 for U0126).