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. Author manuscript; available in PMC: 2015 Oct 30.
Published in final edited form as: J Vaccines Vaccin. 2015 Jun 30;6(3):287. doi: 10.4172/2157-7560.1000287

Figure 6.

Figure 6

Preparation of influenza multi-HA VLPs. (a) Baculovirus expression cassette and schematic composition of multi-HA VLPs [79]. The H5, H7, and H9 genes were derived from A/Viet Nam/1203/2004 (H5N1), A/New York/107/2003 (H7N2) and A/Hong Kong/33982/2009 (H9N2) viruses, respectively. Neuraminidase (NA) and matrix (M1) proteins were derived from A/Puerto Rico/8/1934 (H1N1) virus (shown in gray). (b) Transmission electron microscopy of purified multi-HA VLPs. The VLP preparation was negatively stained with 1% phosphotungstic acid and visualized on a Hitachi H-7600 transmission electron microscope. Size of VLP is indicated. Bar, 100nm.