Table 3. Identification of an optimum condition for CFA/I surface expression using response surface methodology.

Factor	Tested Range	Theoretical range of model	Optimum condition	Variation limits	Unit
PGM	0.1–2.0	0–2.6	2.1	1.3–2.2	g/L
Glucose	5.5–55	0–66.7	44.4	22.2–44.4	mM
Glutamine	0.34–13.7	0–16.4	10.3	6.18–11.0	mM
Lincomycin	10–90	0–102	74.7	67.2–74.8	μg/mL
EGTA	0.1–1.0	0–1.2	0.5	0.5–0.6	mM
FeSO4	5–200	0–228.5	73.4	70.1–87.4	μM
pH	6.0–8.5	5.2–9.3	7.5	6.8–7.7	-