Table 1. Sequence of oligonucleotide primers used in this study.
| Primer name | Oligonucleotide sequence (5’→3’) | Tm(°C) / %GC |
|---|---|---|
| BackboneΔAgeI-F | GCTACTTGTTCTTTTTGCA ACCGGT GGATCCCATCGATTCGAATTC | 70 / 46 |
| Backbone7G-F1 | CCGGT GGCGCGCC GCTAGC GGTGGCGGAGGTGGCGGAGGTTA | 84 / 76 |
| Backbone7G-R1 | CCGGTAACCTCCGCCACCTCCGCCACC GCTAGC GGCGCGCC A | 84 / 76 |
| Backbone7G-F2 | CTAGCGGC ACCGGT GGC TGTACA AGGGAGGCGGTGGAGGCGGTGGG | 82 / 72 |
| Backbone7G-R2 | CTAGCCCACCGCCTCCACCGCCTCCCT TGTACA GCC ACCGGT GCCG | 82 / 72 |
| Backbone7G-F3 | CCGGAGGTGGCGGAGGTGGCGGG ACTAGT CCA GGCGCGCC TCCGC | 84 / 78 |
| Backbone7G-R3 | TCGAGCGGA GGCGCGCC TGG ACTAGT CCCGCCACCTCCGCCACCT | 84 / 73 |
| xERK2.AgeI-BamHI-F | T ACCGGT GGATCC AC ATG GCAGCGGCAGCGGCCTCGTC | 79 / 68 |
| xERK2.XhoI-R | GAGG CTCGAG TCA GTACCCTGGCTGGAATCTAGCG | 71 / 60 |
| eGFP.AscI-F | CGCC GGCGCGCC AGCCATGGTGAGCAAGGGCGAGG | 81 / 77 |
| eGFP.NheI-R | ACC GCTAGC CTTGTACAGCTCGTCCATGCC | 70 / 60 |
| T2A.AscI-F | CGCGCCGG ACTAGT CC ATCGAT GGCAGTGGAGAGGGCAGAGGAAGTCTGCTAACATGCGGTGACGTCGAGGAGAATCCTGGCCCAGGTGG | 84 / 62 |
| T2A.AscI-R | CGCGCCACCTGGGCCAGGATTCTCCTCGACGTCACCGCATGTTAGCAGACTTCCTCTGCCCTCTCCACTGCC ATCGAT GG ACTAGT CCGG | 84 / 62 |
| xMEK1.SpeI-F | GG ACTAGT CCAACATGCCTAAAAAGAAGCCT | 64 / 45 |
| xMEK1.ClaI-F | CC ATCGAT GGCCACTCCGGCGGCATGGGTTG | 74 / 68 |
| mMEK1.SpeI-F1 | GG ACTAGT CCAAGATGCCCAAGAAGAAGCCG | 67 / 55 |
| mMEK1.SpeI-F2 | GG ACTAGT CCCAAGAAGAAGCCGACGCCCATCCAGCTG | 73 / 61 |
| mMEK1.ClaI-R | CC ATCGAT GGCGATGCTGG CAGCGTGGGTTG | 73 / 65 |
| mMEK1.AscI-R | A GGCGCGCC TCA GATGCTGGCAGCGTGGGTTGGTGTGCTGGG | 81/ 69 |
Abbreviations: 7G, 7-glycine linker; F, forward primer; R, reverse primer. Restriction enzyme sites are underlined and start/stop codons are in bold.