Fig 4. siRNA knockdown of 14-3-3ζ expression in HEK 293 cells.
A time dependent plot of levels of 14-3-3ζ expression following siRNA knockdown of the 14-3-3ζ in HEK 293 cells. Cells were seeded at either 1.0 or 2.0 x106cells/well in 6-well culture dishes. For 14-3-3ζ targeting, three independent Stealth RNA duplex primers (Life Technologies) were pooled and used at a final concentration of 100 nM. Residual 14-3-3ζ expression levels were determined at 48, 72 and 96 h post transfection. Beneath the plots are Western blots showing the extent of 14-3-3ζ depletion (optimal at 72 h) at 1.0 x 106 cells/well.