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. 2015 Oct 30;10(10):e0141968. doi: 10.1371/journal.pone.0141968

Fig 6. Non-reducing 2D-GE Western blot analyses of ASK1 signaling complex in HEK 293 cells.

Fig 6

A non-reducing 2D-GE that preserves endogenous disulfide linkage was performed on Klotho-treated and buffer-control cells as described in the text. Images in A (buffer treated) or B (Klotho treated) are representative Western blots probed with either Trx antibody or stripped and re-probed with (i) ASK1 and/or (ii) 14-3-3ζ. Closed circled spots indicate free Trx and/or 14-3-3ζ proteins based on pI and/or molecular size. The dotted rectangular spots represent ASK1-bound complexes with Trx and/or 14-3-3ζ and other proteins cross-reactive with the Trx antibody that were more abundant in Klotho treated cells than in the buffer control.