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. 2015 Oct 30;10(10):e0141546. doi: 10.1371/journal.pone.0141546

Fig 1. Motility assays of Bacillus subtilis wild type and mutant strains.

Fig 1

Inoculums are placed in the center of 0.3% agar plates, and spreading is analyzed after 18 hours of incubation. A) wild type, B) ΔsigD = sigma D deletion (DS313), C) ΔbacEF = deletion of bactofilin genes (JEA100), D) ΔbacEF EF compl = deletion of bactofilin genes, expression of bacE and bacF from the amylase locus (JEA111), E) deletion of bactofilin genes, expression of bacE from the amylase locus (JEA134), F) deletion of bactofilin genes, expression of bacF from the amylase locus (JEA135), G) YFP-BacE (JEA102), and H) YFP-BacF (JEA104), I) BacE-YFP (JEA101), J) BacF-YFP (JEA103), K) expression of bacE-yfp from the amylase locus in wild type cells (JEA130), L) expression of bacF-yfp from the amylase locus in wild type cells (JEA131), M) expression of YFP-BacE from the amylase locus in the bacEF deletion strain (JEA112), N) FliM-CFP (JEA107), O) YFP-FliG (JEA105), P) YFP-FliY (JEA132).