Table I.
Markers expressed by mouse and primate TNP-specific B cells before and after immunizationa
| Phenotypic Marker | Naive | Immune (day 5) | ||
|---|---|---|---|---|
| Mice | Primates | Mice | Primates | |
| Forward scatter (FSC) | Low | Low | High | High |
| Side scatter (SSC) | Low | Low | High | High |
| CD11b (Mac-1/CR3) | − | − | + | + |
| CD19 | + | + | High | High |
| CD21 | + | + | Low | Low |
| CD20 | + | + | ? | Low |
| B220 | + | ? | Low | ? |
| PD-1 | − | − | + | + |
| CD86 | − | − | + | + |
| CD80 | − | − | + | + |
| CD44 | − | − | + | + |
| CD43 | − | ? | + | ? |
| CD23 | + | ? | − | ? |
| CD27 | − | − | − | + |
a
Data is derived from references 12 and 52 and represent the phenotype of mouse spleen B cells or primate blood B cells before and 5 to 7 days after immunization with TNP-Ficoll (immunization was intraperitoneal for mice and intraveneous for African Green monkeys and cynomolgus macaques). It should be noted that not all TNP-specific B cells express changes in markers following immunization, but that these alterations are due to the presence of cells expressing CD11b and increased size (FSChi).