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. 2015 Oct 27;3:e1351. doi: 10.7717/peerj.1351

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© 2015 Kilbride et al.

This is an open access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, reproduction and adaptation in any medium and for any purpose provided that it is properly attributed. For attribution, the original author(s), title, publication source (PeerJ) and either DOI or URL of the article must be cited.

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Comparing the effects of cyclodextrin treatment on the biochemical composition of buoyant and dense membrane fractions isolated on equilibrium sucrose density gradients. (A) Change in cholesterol levels as determined by Amplex Red cholesterol assays. Note that there was no significant change in the total amount of cholesterol present in the dense membranes. (B) Levels of the membrane—associated protein PI4KIIα were determined by Western blotting and quantitated by image analysis software. Cyclodextrin addition causes a redistribution of PI4KIIα from the buoyant to the dense fractions. Results are presented as mean ± S.E.M from experiments repeated three times, ^∗∗∗p < 0.001, ^∗∗p < 0.01, NS not statistically significant using the two-tailed student t-test.