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. 2015 Oct 26;9(5):054124. doi: 10.1063/1.4934713

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© 2015 Author(s).

1932-1058/2015/9(5)/054124/15

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FIG. 3. — ZO-1 staining of HBMVECs in microfluidic channels under flow and under different culture conditions. (a) Control culture using EBM-2 media, showing higher intensity ZO-1 staining around the cell border that indicates tight junction staining. (b) Astrocyte-conditioned EBM-2 media, showing a trend towards increased tight junctions. (c) Pericyte-conditioned EBM-2 media, showing significant increases in staining around the cell border (p = 0.002, N = 10). (d) EBM-2 media with no serum, showing that the absence of serum reduces cell survival. (e) Mean intensity of ZO-1 staining at cell borders. 10 cells for each condition were evaluated and could not share a border with a cell that had already been counted.