. 2015 Oct 13;4:600. doi: 10.1186/s40064-015-1369-9

Table 3.

Media composition for the transformation and regeneration of the japonica rice varieties Curinga and Nipponbare and the indica rice variety IR-64

Medium code	Use	Composition
CHU-Ind	Induction and proliferation of calli.	N6 salts and vitamins (Chu 1978), 100 mg l⁻¹ Myo-inositol, 2.5 mg l⁻¹ 2,4-d, 300 mg l⁻¹ Casein enzymatic hydrolysate, Maltose 30 g l⁻¹, Gelrite 3 g l⁻¹. Autoclave and supplement l-proline 500 mg l mg l⁻¹ and l-glutamine 500 mg l⁻¹. pH 5.8
CHU-Ind-AS	Co-cultivation	Chu-Ind medium supplemented with 100 µM acetosyringone. pH 5.8.
CHU-infection	Agro-Infection	N6 salts and vitamins (Chu 1978), 100 mg l⁻¹ Myo-inositol, 2,5 mg l⁻¹ 2,4-d, 1 g l⁻¹ Casamino acids, Maltose 15 g l⁻¹, Glucose 15 g l⁻¹. pH: 5.2. Filter sterilize. Add sterile acetosyringone 100 µM
CHU-H20 CHU-H40 CHU-H30	Selection	Chu-Ind medium supplemented with l-proline 500 mg l⁻¹, l-glutamine 500 mg l⁻¹, Gelrite 3 g l⁻¹, 20 mg l⁻¹ Hygromycin (for Curinga), 40 mg l⁻¹ Hygromycin (for Nipponbare), 30 mg l⁻¹ Hygromycin (for IR64), 250 mg l⁻¹ Cefotaxime. pH 5.8
MS-H20 MS-H40 MS-H30	Shoot induction/regeneration	MS salts and vitamins (Murashinge and Skoog 1962), 100 mg l⁻¹ Myo-inositol, 1 mg l⁻¹ NAA, 30 g l⁻¹ Sucrose, 3 g l⁻¹ Gelrite. Autoclave and supplement with 4 mg l⁻¹ Kinetin, 20 mg l⁻¹ Hygromycin (for Curinga), 40 mg l⁻¹ Hygromycin (for Nipponbare), 30 mg l⁻¹ Hygromycin (for IR64), and 250 mg l⁻¹ Cefotaxime. pH 5.8
MS-R-20 MS-R-40 MS-R-30	Rooting	MS salts and vitamins. 30 g l⁻¹ Sucrose, 3 g l⁻¹ Gelrite. Autoclave and supplement with, 20 mg l⁻¹ Hygromycin (for Curinga), 40 mg l⁻¹ Hygromycin (for Nipponbare), 30 mg l⁻¹ Hygromycin (for IR64), 250 mg l⁻¹ Cefotaxime. pH 5.8