FIG 6.

Pak2 regulates cytoskeletal organization and remodeling. (A) HUVECs were sparsely plated on fibronectin-coated slides, fixed, and stained with phalloidin and DAPI. (B) Sparsely plated HUVECs were fixed and stained with pMLC antibody and phalloidin. Arrows indicate phosphorylated myosin light chain (pMLC) enrichment at the sides and back of the cell, and asterisks indicate cells with abundant centrally localized pMLC signal. (C) HUVECs were plated to confluence on fibronectin-coated slides, fixed, and stained with DAPI and VEC and β-catenin antibodies. (D) Confluent HUVECs fixed and stained with phalloidin. (E) HUVECs were plated to confluence, allowed to form cell-cell junctions, stimulated with vehicle control or 10 μM forskolin for 20 min, fixed, and stained with phalloidin and Pak2 and β-catenin antibodies. Images are representative of three independent experiments.