Fig. 4.

Variable nucleotide tandem repeat (VNTR) and Coomassie SDS PAGE analyses of Yarrowia lipolytica wild-type and mutant strains A, B, B2, C, D, E, and F. a Genomic DNA extracted from strains grown in LNG medium was amplified by PCR using the VNTR oligonucleotide indicated at upper right and the amplified DNA loaded onto 1 % (w/v) agarose gels stained with ethidium bromide. Marker in lanes labeled M is lambda DNA-Hind III/phiX-174 RF DNA-Hae III (72 bp to 23 kb); Y. lipolytica mutant strains are in lanes labeled B2, A, B, C, D, E, and F; wild-type strain is in lane WT. b Cell protein profile of samples prepared from cultures grown in YPD medium. Samples were loaded onto a 4 to 20 % tris-glycine SDS-PAGE gel and gel was stained with Coomassie Brilliant Blue R-250. Lane M is SeeBlue® Plus2 marker; Y. lipolytica mutant strains are in lanes labeled B2, A, B, C, D, E, and F; wild-type strain is in lane WT