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. 2015 May 24;73(3):609–617. doi: 10.1007/s12013-015-0630-8
Reagents and instruments Company
DMEM/F-12 medium, fetal bovine serum (FBS) Thermo, USA
β-glycerophosphate, dexamethasone, ascorbic acid, Recombinant human CGRP, sodium alginate Sigma USA
Trypsin, collagenase type I, MTT cell proliferation and cytotoxicity detection kit Beyotime institute of Biotechnology
Rabbit Anti-CD44/CD45 Beijing Biosynthesis Biotechnology CO., Ltd.
FITC-labeled Goat anti-rabbit IgG Beijing ZSGB-BIO
ALP kit, ALP staining kit, Calcium assay kit Nanjing Jiangcheng Bioengineering Institute
Total RNA extraction kit, RT kit, PCR reaction liquid TIANGEN BIOTECH (Beijing) CO., LTD
Laminar flow hood Suzhou Purification Equipment Co., LTD
Phase-contrast microscope Olympus Optical Co., Ltd
Enzyme-linked immunosorbent assays Toshiba (Japan) Co., Ltd.
Fluorescence microscopy Nikon (Japan) Co., Ltd.
PCR Instrument MJ Research Co., Ltd.
UV Gel imaging system Alpha Innotech Co., Ltd.