DMEM/F-12 medium, fetal bovine serum (FBS) |
Thermo, USA |
β-glycerophosphate, dexamethasone, ascorbic acid, Recombinant human CGRP, sodium alginate |
Sigma USA |
Trypsin, collagenase type I, MTT cell proliferation and cytotoxicity detection kit |
Beyotime institute of Biotechnology |
Rabbit Anti-CD44/CD45 |
Beijing Biosynthesis Biotechnology CO., Ltd. |
FITC-labeled Goat anti-rabbit IgG |
Beijing ZSGB-BIO |
ALP kit, ALP staining kit, Calcium assay kit |
Nanjing Jiangcheng Bioengineering Institute |
Total RNA extraction kit, RT kit, PCR reaction liquid |
TIANGEN BIOTECH (Beijing) CO., LTD |
Laminar flow hood |
Suzhou Purification Equipment Co., LTD |
Phase-contrast microscope |
Olympus Optical Co., Ltd |
Enzyme-linked immunosorbent assays |
Toshiba (Japan) Co., Ltd. |
Fluorescence microscopy |
Nikon (Japan) Co., Ltd. |
PCR Instrument |
MJ Research Co., Ltd. |
UV Gel imaging system |
Alpha Innotech Co., Ltd. |